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Effect of transforming growth factor β on fibroblasts in three‐dimensional lattice cultures
Author(s) -
Coustry Françoise,
Gillery Philippe,
Maquart François-Xavier,
Borel Jacques-Paul
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80223-6
Subject(s) - transforming growth factor , fibrin , chemistry , biophysics , growth factor , monolayer , microbiology and biotechnology , biochemistry , biology , immunology , receptor
Human skin fibroblasts were cultivated in three‐dimensional fibrin or collagen lattices, under retracting or non‐retracting conditions, and the influence of transforming growth factor β (TGFβ) was tested. TGFβ stimulated the synthesis of non‐collagen protein and of collagen in all the systems. However, only in non‐retracting fibrin lattices did it restore a level of protein synthesis comparable to that found in monolayers. The effects of TGFβ greatly depended on the type of substratum and on the presence or absence of retraction.