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Functional expression of Ca 2+ ‐mobilizing α‐thrombin receptors in mRNA‐injected Xenopus oocytes
Author(s) -
Van Obberghen-Schilling Ellen,
Chambard Jean Claude,
Lory Philippe,
Nargeot Joel,
Pouysségur Jacques
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80221-4
Subject(s) - xenopus , messenger rna , receptor , thrombin , microbiology and biotechnology , chemistry , biology , biochemistry , gene , platelet , immunology
α‐Thrombin (TH) initiates a program of intracellular events that lead to DNA replication in quiescent CCL39 Chinese hamster lung fibroblasts via membrane receptors that have yet to be characterized at a molecular level. Functional TH receptors were expressed in Xenopus laevis oocytes following injection of poly(A) + RNA from TH‐responsive CCL39 cells; their presence was demonstrated by TH‐stimulated 45 Ca 2+ efflux or Ca 2+ dependent Cl − channel activation. In voltage clamp experiments on microinjected oocytes a Ca 2+ ‐activated Cl − current was detected in response to TH (0.2–10). The TH response was blocked by a specific TH inhibitor, and potentiated by addition of FGF or intracellular injection of GTP‐γ‐S.