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Competitive binding of the troponin T‐specific pool of caldesmon antibodies and tropomyosin to skeletal troponin T and smooth muscle caldesmon
Author(s) -
Birukov Konstantin G.,
Shirinsky Vladimir P.,
Vorotnikov Alexander V.,
Gusev Nikolai B.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80206-x
Subject(s) - caldesmon , tropomyosin , polyclonal antibodies , troponin t , troponin c , troponin , epitope , microbiology and biotechnology , actin , skeletal muscle , biochemistry , chemistry , antibody , gene isoform , biology , calmodulin , medicine , immunology , endocrinology , enzyme , gene , myocardial infarction
The fraction of polyclonal caldesmon antibodies cross‐reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in M r 77 kDa non‐muscle caldesmon. These results provide functional confirmation for the suggestion that the regions of amino acid sequence homology in caldesmon isoforms and troponin T belong to the tropomyosin binding sites.