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Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin
Author(s) -
Man Nguyen thi,
Cartwright A.J.,
Morris G.E.,
Love D.R.,
Bloomfield J.F.,
Davies K.E.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80199-s
Subject(s) - immunogen , monoclonal antibody , microbiology and biotechnology , epitope , fusion protein , dystrophin , antibody , recombinant dna , muscular dystrophy , duchenne muscular dystrophy , epitope mapping , biology , chemistry , biochemistry , immunology , genetics , gene
Nineteen monoclonal antibodies which bind to native dystrophin in the plasma membrane of frozen muscle sections were obtained using a recombinant fusion protein as immunogen. On Western blots of normal mouse muscle extracts, the antibodies bind specifically to a 400 000 M r protein which is absent from dystrophic mouse ( mdx ) muscle. At least four distinct epitopes have been identified by cleavage mapping methods. Although the fusion protein contained 25% of the human dystrophin sequence (Cys 816 ‐Asp 1747 ; M r 108 000), most of the monoclonal antibodies (15 out of 19) recognize a single fragment of M r 27 500.