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Glycolysis compared in intact, permeabilized and sonicated L‐929 cells
Author(s) -
Jackson Susan A.,
Thomson Marilyn J.,
Clegg James S.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80192-l
Subject(s) - glycolysis , biochemistry , enzyme , cell , chemistry , incubation , biology , microbiology and biotechnology , biophysics
The effects of incubation time and cell density on glycolytic rate were examined in suspensions of intact, permeabilized and sonicated L‐929 cells. Sonicates exhibited strong dependence on cell density and a distinct lag in glycolytic rate, while intact cells showed no cell density dependence and linear glycolytic rates. Permeabilized cells exhibited linear glycolytic rates, but sometimes showed dependence on cell density. Rates of lactate production (nmol at 30 min/10 6 cells) were highest in sonicates and lowest in intact cells. These results are interpreted as support for the previously proposed hypothesis that enzymes of the glycolytic pathway are highly organized in intact L‐929 cells.