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Identification of a group of proteins that are strongly up‐regulated in total epidermal keratinocytes from psoriatic skin
Author(s) -
Celis Julio E.,
Cruger Dorthe,
Kiil Jette,
Lauridsen Jette B.,
Ratz Gitte,
Basse Bodil,
Celis Ariana
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80179-m
Subject(s) - keratinocyte , psoriasis , isoelectric focusing , biology , two dimensional gel electrophoresis , pathogenesis , gel electrophoresis , microbiology and biotechnology , epidermis (zoology) , polyacrylamide gel electrophoresis , keratin , biochemistry , immunology , proteomics , enzyme , in vitro , genetics , gene , anatomy
Analysis using two‐dimensional (2D) gel electrophoresis of the [ 35 S]‐methionine‐labelled proteins synthesized by non‐cultured total epidermal keratinocytes obtained from normal and psoriatic skin revealed 6 proteins that are strongly up‐regulated (5 times or more) in psoriatic skin. These proteins are synthesized at albeit lower levels by keratinocytes from normal and normal‐appearing (uninvolved) skin of psoriatic patients, and correspond to isoelectric focusing sample spot numbers 4311 (40.3 kDa), 4003 (12.4 kDa), 5008 (11.9 kDa), 3012 (11.6 kDa), 6016 (11.6 kDa) and 1015 (10;1 kDa) in the normal keratinocyte 2D gel protein database [Celis et al, (1990) Electrophoresis, in press]. These proteins are also detected in the labelling medium indicating that they are at least in part secreted. Given their striking regulatory behavior, these proteins may play a role in the pathogenesis of psoriasis.