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Molecular cloning of cDNA for vitamin D 3 25‐hydroxylase from rat liver mitochondria
Author(s) -
Usui Emiko,
Noshiro Mitsuhide,
Okuda Kyuichiro
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80172-f
Subject(s) - complementary dna , cdna library , microbiology and biotechnology , open reading frame , peptide sequence , biology , clone (java method) , enzyme , biochemistry , cloning (programming) , amino acid , nucleic acid sequence , mitochondrion , gene , programming language , computer science
A cDNA clone encoding mitochondrial vitamin D 3 25‐hydroxylase was isolated from a rat liver cDNA library by the use of specific antibodies to the enzyme. The isolated cDNA clone was 1.9 kbp long and contained a 1599 bp open reading frame encoding 533 amino acid residues. The deduced primary structure contained a presequence typical for mitochondrial enzymes in the N‐terminal region. The N‐terminal sequence of the mature enzyme was determined to be Ala‐Ile‐Pro‐Ala‐Ala, which agrees perfectly with a portion of the deduced sequence, establishing the cleavage point of the precursor.