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The ribonuclease from an extinct bovid ruminant
Author(s) -
Stackhouse Joseph,
Presnell Scott R.,
McGeehan Gerard M.,
Nambiar Krishnan P.,
Benner Steven A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80164-e
Subject(s) - ribonuclease , ruminant , biology , most recent common ancestor , ancestor , mutagenesis , swamp , sequence (biology) , phylogenetics , evolutionary biology , genetics , rna , mutation , ecology , gene , geography , archaeology , crop
The sequence of the ribonuclease from the ancestor of swamp buffalo, river buffalo, and ox, corresponding approximately to Pachyportax latidens , an extinct ruminant known from the fossil record, has been reconstructed using the rule of ‘maximum parsimony’. This protein and two sequences that may have been intermediates in the evolution of modern ribonuclease have been constructed in the laboratory by site‐directed mutagenesis, and their properties examined.