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A 43 kDa form of the GTP‐binding protein G i3 in human erythrocytes
Author(s) -
Carty Donna J.,
Iyengar Ravi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80163-d
Subject(s) - heterotrimeric g protein , antiserum , g protein , protein subunit , pertussis toxin , biology , binding protein , gtp' , biochemistry , gtp binding protein regulators , microbiology and biotechnology , g beta gamma complex , peptide sequence , antibody , enzyme , genetics , gene , receptor
Purified preparations of human erythrocyte G‐proteins contain a 43 kDa pertussis toxin substrate which appears to be the α‐subunit of a heterotrimeric GTP‐binding protein. The 43 kDa protein is recognized by antisera that are sequence‐specific for peptides encoding a sequence common to all 39–53 kDa G‐protein α‐subunits. Gα o ‐specific antiserum did not recognize 43 or 40–41 kDa α‐subunits. AS/6, which recognizes the α i proteins, recognized 43 kDa as well as 40–41 kDa proteins. Of the three antisera specific for individual members of the α i family, only the G i3 ‐specific antiserum recognized the 43 kDa erythrocyte G‐protein. However, 40–41 kDa forms of all three α i s are present. These observations indicate that human erythrocytes contain a novel 43 kDa form of G i3 .