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Phorbol 12‐myristate 13‐acetate (PMA) increases the expression of the nerve growth factor (NGF) gene in mouse L‐929 fibroblasts
Author(s) -
Wion Didier,
Grogan Donal Mac,
Houlgatte Rémi,
Brachet Philippe
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80149-d
Subject(s) - cycloheximide , nerve growth factor , protein kinase c , phorbol , messenger rna , downregulation and upregulation , divalent , gene expression , phorbol ester , microbiology and biotechnology , ionophore , protein kinase a , chemistry , protein kinase inhibitor , protein biosynthesis , gene , endocrinology , biology , biochemistry , kinase , receptor , organic chemistry , membrane
The rise of the NGF mRNA pool which takes place following exposure of L‐929 fibroblasts to serum was prevented in the presence of 5 μM K‐252a, a compound which inhibits several species of protein kinase activities. To characterize further this phenomenon, L‐929 cells growing in a serum‐free medium were exposed to cyclic nucleotide analogs, to a divalent cation ionophore or to the phorbol ester PMA. Only this latter compound induced an enhancement of the NGF mRNA pool, suggesting an involvement of protein kinase C in the upregulation of the NGF transcripts. The effects of PMA or serum also require a synthesis of protein since the level of NGF transcripts remained stable in the presence of cycloheximide.