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Molecular cloning of two distinct vasotocin precursor cDNAs from chum salmon ( Oncorhynchus keta ) suggests an ancient gene duplication
Author(s) -
Heierhorst Jörg,
Mahlmann Stefan,
Morley Steven D.,
Coe Imogen R.,
Sherwood Nancy M.,
Richter Dietmar
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80129-7
Subject(s) - oncorhynchus , vasotocin , cloning (programming) , biology , amino acid , gene , molecular cloning , peptide sequence , gene duplication , microbiology and biotechnology , biochemistry , fish <actinopterygii> , fishery , neuropeptide , receptor , computer science , programming language
The structures of two different vasotocin precursors from chum salmon brain have been elucidated through the molecular cloning of their corresponding cDNAs. Although the predicted precursors, consisting respectively of 153 and 158 amino acids, have the same structural organisation, they show 35% amino acid sequence divergence, of which only approximately half are isofunctional substitutions. Remarkably, while the C terminal segments of both precursors resemble the glycopeptide moiety of the related mammalian vasopressin precursor, both salmon precursors lack consensus sequences for N‐glycosylation.