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ESR signals of NADH and NADPH under illumination
Author(s) -
Umrikhina A.V.,
Luganskaya A.N.,
Krasnovsky A.A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80127-5
Subject(s) - radical , nad+ kinase , chemistry , cofactor , photochemistry , electron paramagnetic resonance , action spectrum , enzyme , nuclear magnetic resonance , biochemistry , physics
ESR method was applied to investigate the formation of NADH and NADPH free radicals. It was shown that under the action of light (340 nm) in water and other solutions of these compounds a reaction occurred resulting in the formation of free radicals having the typical ESR spectrum. The analysis of the temperature dependence showed the light‐induced ESR signals to be registered at −30°C to −120°C, the most intensive ones being observed at −50°C. It was concluded that the observed ESR signals belonged to the products of one‐electron oxidation of the coenzymes‐free radicals NAD . and NADP . .