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Expression of protein kinase C I in NIH 3T3 cells increases its growth response to specific activators
Author(s) -
Cuadrado Antonio,
Molloy Christopher J.,
Pech Michael
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80123-z
Subject(s) - protein kinase c , 3t3 cells , microbiology and biotechnology , tetradecanoylphorbol acetate , biology , cell growth , protein kinase a , fibroblast , signal transduction , cell culture , chemistry , kinase , transfection , biochemistry , gene , genetics
In order to investigate the effects of protein kinase C (PKC) expression on cellular growth and morphology, we established mouse fibroblast cell populations which expressed the rat pkc ‐γ gene under the control of a retroviral promoter. NIH 3T3 stable transfectants displayed a three‐fold increase in total PKC levels. These cells appeared morphologically unaltered but exhibited a stronger mitogenic response to 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA) and cardiolipin (CL) as well as enhanced growth in semisolid medium in the presence of TPA. Thus, at these enzyme levels, PKC conferred growth advantages to NIH 3T3 cells only in response to specific activators.