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Specific interaction of the new fluorescent dye 10‐ N ‐nonyl acridine orange with inner mitochondrial membrane
Author(s) -
Maftah Abderrahman,
Petit Jean Michel,
Julien Raymond
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80112-v
Subject(s) - acridine orange , cardiolipin , fluorescence , mitochondrion , chemistry , acridine , biochemistry , inner mitochondrial membrane , electron transport chain , biophysics , membrane , membrane fluidity , biology , phospholipid , organic chemistry , apoptosis , quantum mechanics , physics
The fluorescent dye 10‐ N ‐nonyl acridine orange (NAO), known as specifically associated with mitochondria, has been reported to have a cytotoxic effect when high doses were applied to cells. Presently, the biochemical basis of its toxicity was investigated on isolated rat liver mitochondria. At low concentrations, NAO strongly inhibited state 3 respiration and ATP synthesis. At high concentrations, electron transport, ATP hydrolysis, P i ‐transport and adenine nucleotide activities were also decreased. All these inhibitions can be explained by probe‐cardiolipin interactions which could induce the collapse of energy conversion and/or the modification of membrane fluidity.