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Interactions between nitrogen oxide‐containing compounds and peripheral benzodiazepine receptors
Author(s) -
Weissman Ben A.,
Bolger Gordon T.,
Chiang Peter K.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80095-z
Subject(s) - sodium nitroprusside , pharmacology , gabaa receptor , long term potentiation , chemistry , receptor , nitric oxide , dihydropyridine , calcium , medicine , endocrinology , biology , biochemistry
Nitrogen oxide‐containing compounds displaced the peripheral benzodiazepine ligand [ 3 H]Ro5‐4864 from guinea pig membrane preparations. Sodium nitroprusside (SNP) was the most potent ( IC 50 = 5.61 ± 1.72 × 10 −5 M). Moreover, its ability to bind to these receptors showed marked tissue variability (heart > kidney ⪢ cerebral cortex). When tested on rat atrium, SNP by itself had no effect on basal inotropy or the increase in inotropy induced by (−)‐S‐BAY K 8644. In contrast, Ro5‐4864 potentiated the marked increase in inotropy induced by (−)‐S‐Bay K 8644, and SNP completely abolished the potentiation of inotropy observed with Ro5‐4864. Since peripheral benzodiazepine receptors are associated with calcium mobilization in the heart, these findings may indicate that some of the clinical effects of nitric oxide‐generating drugs could be mediated by these receptors.