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Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells
Author(s) -
Yamauchi Daisuke,
Minamikawa Takao
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80083-u
Subject(s) - escherichia coli , coding region , gene , biology , microbiology and biotechnology , concanavalin a , signal peptide , intron , peptide sequence , cleavage (geology) , genetics , in vitro , paleontology , fracture (geology)
We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata . The sequence covers the whole transcribed region as well as the 5'‐ and 3'‐untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G‐50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation.