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Calcium‐dependent phospholipid binding proteins associated with the membranes of rabbit skeletal muscle
Author(s) -
Melgunov Vladimir I.,
Mamedova Nigyar A.,
Akimova Elena I.,
Adzhimolaev Tarikh A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80070-y
Subject(s) - egta , phosphatidic acid , phospholipid , phosphatidylserine , chemistry , liposome , biochemistry , endoplasmic reticulum , phosphatidylcholine , calcium , membrane , phosphatidylethanolamine , biophysics , biology , organic chemistry
By using extraction in the presence of Ca 2+ and Triton X‐100 and then in the presence of EGTA without detergent, a set of Ca 2+ ‐dependent phospholipid binding proteins has been identified in the membranes of transverse tubules (T‐tubules) and sarcoplasmic reticulum (SR), isolated from rabbit skeletal muscles. Longitudinal SR, junctional SR and T‐tubule membranes yielded about 9, 14 and 3.3 μg of EGTA‐soluble proteins per 1 mg of membrane protein, respectively. In the presence of 1 mM CaCl 2 , 68 and 33 kDa proteins of T‐tubules and junctional SR as well as 30 kDa protein of T‐tubules were shown to bind to liposomes made of 1:1mixtures of (i) phosphatidylcholine and (ii) phosphatidylserine, phosphatidic acid, or phosphatidyl ethanolamine. In the presence of EGTA, the above‐mentioned proteins were mostly found in the supernatants. Binding of the proteins with liposomes consisting of pure phosphatidylcholine was negligible.