Premium
Heterogeneity of protein kinase C expression and regulation in T lymphocytes
Author(s) -
Lucas S.,
Marais R.,
Graves J.D.,
Alexandere D.,
Parker P.,
Cantrell D.A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80064-p
Subject(s) - jurkat cells , protein kinase c , lymphoblast , microbiology and biotechnology , t cell , chemistry , phosphorylation , biology , cell culture , immunology , genetics , immune system
The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC α,β 1 , and β 2 and γ, it was observed that T cells expressed two PKC isotypes, PKC α and β. No PKC γ was detected in T lymphocytes. In lymphoblasts, high levels of PKC β compared to PKC α were found whereas Jurkat cells expressed high levels of α compared to PKC β. Differences in the calcium sensitivity of phorbol ester‐induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC α and β isotypes expressed by the cells.