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Stimulation of protein synthesis by raised extracellular pH in cardiac myocytes and perfused hearts
Author(s) -
Gaitanaki Catherine J.,
Sugden Peter H.,
Fuller Stephen J.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80061-m
Subject(s) - stimulation , myocyte , extracellular , chemistry , medicine , microbiology and biotechnology , biophysics , endocrinology , biochemistry , biology
Protein synthesis was stimulated in freshly‐isolated rat cardiac myocytes by increasing the extracellular pH of Hepes‐buffered Tyrode's solutions over the range pH 7.4–8.4. The maximal stimulation was about 45%. Protein synthesis in anterogradely‐perfused rat hearts was stimulated by 11% by increasing the pH of the bicarbonate‐containing perfusion medium from pH 7.4 to 7.8. This manoeuvre increased intracellular pH by 0.12 units. A concomitant increase in phosphocreatine concentration was observed. These findings are consistent with the hypothesis that intracellular pH may exert profound effects on tissue protein synthesis rates.