Premium
High molecular weight forms of basic fibroblast growth factor recognized by a new anti‐bFGF antibody
Author(s) -
Grothe Claudia,
Zachmann Karolin,
Unsicker Klaus,
Westermann Reiner
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80059-r
Subject(s) - basic fibroblast growth factor , antibody , western blot , ovary , microbiology and biotechnology , biology , peptide , pituitary gland , blot , chemistry , growth factor , biochemistry , endocrinology , hormone , immunology , receptor , gene
An antibody against basic fibroblast growth factor (bFGF) was raised using purified bovine pituitary bFGF. Western blot analysis revealed immunoreactive bands at 18, 24, 30–33 and 46 kDa in inununoaffinity purified extracts of pituitary and adrenal gland using this antibody. A similar staining pattern was obtained with ovary extracts with the exception of the missing 18 kDa band. A second anti‐bFGF antibody raised against a synthetic peptide comprising the 24 N‐terminal amino acids of bFGF reacted with the 18 kDa and the 46 kDa band of immunoaffinity purified ovary and adrenal gland extracts.