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Photolabile μ‐conotoxins with a chromogenic phenyldiazirine
Author(s) -
Hatanaka Yasumaru,
Yoshida Eiichi,
Nakayama Hitoshi,
Abe Teruo,
Satake Mei,
Kanaoka Yuichi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80057-p
Subject(s) - chromogenic , reagent , chemistry , saxitoxin , sodium , macromolecule , photoaffinity labeling , sodium channel , combinatorial chemistry , chromatography , toxin , biophysics , binding site , biochemistry , organic chemistry , biology
Three photoreactive derivatives of μ‐conotoxin G IIIA have been prepared as photoaffinity labeling reagents for muscle‐type sodium channels. The reagents competitively inhibited the binding of saxitoxin to the eel sodium channel with K i values of 11–18 nM. The introduced chromogenic phenyldiazirine group on the toxin was photolyzed efficiently, and spectroscopic properties of the reagents demonstrated that irradiation and detection can be performed in a spectral region where the absorptions due to most of biological macromolecules are negligible.