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Extensive precursor‐product relationship between gangliosides formed from exogenous glucosylceramide in rat liver
Author(s) -
Trinchera Marco,
Vallenzasca Carlo,
Ghidoni Riccardo,
Tettamanti Guido
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80056-o
Subject(s) - ganglioside , biochemistry , residue (chemistry) , endogeny , chemistry , biosynthesis , ceramide , enzyme , apoptosis
Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor‐product relationship actually exists in the course of ganglioside biosynthesis.