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PAPS‐reductase of Escherichia coli
Author(s) -
Krone Frank A.,
Westphal Goetz,
Meyer Helmut E.,
Schwenn Jens D.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80052-k
Subject(s) - escherichia coli , open reading frame , biochemistry , gene , structural gene , reductase , thioredoxin reductase , peptide sequence , enzyme , 7 dehydrocholesterol reductase , gene product , biology , thioredoxin , amino acid , microbiology and biotechnology , chemistry , gene expression
The DNA of the gene complementing a PAPS‐reductase‐deficient strain of Escherichia coli was sequenced. The N‐terminal amino acid sequence of the purified PAPS‐reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of M r = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme.