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Isolation and sequence determination of human brain natriuretic peptide in human atrium
Author(s) -
Kambayashi Yoshikazu,
Nakao Kazuwa,
Mukoyama Masashi,
Saito Yoshihiko,
Ogawa Yoshihiro,
Shiono Shozo,
Inouye Ken,
Yoshida Nobuo,
Imura Hiroo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80043-i
Subject(s) - peptide sequence , peptide , npr2 , sequence (biology) , natriuretic peptide , brain natriuretic peptide , medicine , signal peptide , atrium (architecture) , human brain , sequence analysis , atrial natriuretic peptide , complementary dna , npr1 , endocrinology , chemistry , biology , microbiology and biotechnology , biochemistry , gene , atrial fibrillation , heart failure , neuroscience
We isolated human brain natriuretic peptide (human BNP) from the human atrium. Sequence analysis has revealed that it is a 32‐amino‐acid peptide with the sequence S‐P‐K‐M‐V‐Q‐G‐S‐G‐C‐F‐G‐R‐K‐M‐D‐R‐I‐S‐S‐S‐S‐G‐L‐G‐C‐K‐V‐L‐R‐R‐H, which is identical to the C‐terminal sequence (77–108) of the human BNP precursor deduced from the cDNA sequence. The sequence of human BNP (77–108) is preceded by Pro 75 ‐Arg 76 in the human BNP precursor, which is the same processing signal as Pro 97 ‐Arg 98 of the precursor of atrial natriuretic peptide (ANP). The processing of the BNP precursor occurs in the cardiocyte, although that of the ANP precursor in the cardiocyte is unclear at present.