Premium
A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus‐specific proteins in MDCK cells
Author(s) -
Kabanov Alexander V.,
Vinogradov Sergei V.,
Ovcharenko Alexander V.,
Krivonos Alexander V.,
Melik-Nubarov Nikolai S.,
Kiselev Vsevolod I.,
Severin Eugenii S.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80039-l
Subject(s) - virus , oligonucleotide , polymerase , influenza a virus , biology , rna , virology , dna , chemistry , microbiology and biotechnology , biochemistry , gene
To enhance the penetration of oligonucleotide (‘oligo’) into cells, the oligo was combined with the hydrophobic undecyl residue. Using the ‘DNA‐synthesator’, we synthesized oligo, complementary to the loop‐forming site of the RNA, encoding polymerase 3 of the influenza virus (type A), and combined it with the undecyl residue added to the 5' terminal phosphate group. It was found that the modified oligo effectively suppresses the influenza A/PR8/34 (H1N1) virus reproduction and inhibits the synthesis of virus‐specific proteins in MDCK cells. Under the same conditions, the non‐modified antisense oligo and modified nonsense oligo did not affect the virus development.