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Fluorescence properties of the Ca 2+ Mg 2+ ‐ATPase protein of sarcoplasmic reticulum labeled with 7‐chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐ diazole
Author(s) -
Bailin Gary,
Huang John R.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80021-a
Subject(s) - endoplasmic reticulum , chemistry , fluorescence , atpase , calcium , fluorophore , calcium atpase , biophysics , biochemistry , enzyme , biology , physics , organic chemistry , quantum mechanics
The fluorescence intensity of the Ca 2+ ,Mg 2+ ‐ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7‐chloro‐4‐nitrobenzo‐2‐oxa‐1, 3‐diazole (NBD‐Cl) was enhanced at high MgATP concentrations with or without 50 μM calcium. The observed enhancement indicates that the fluorophore, NBD‐Cl, can detect conformational changes in the ATPase protein.