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Monoclonal antibody to galactosylceramide: discrimination of structural difference in the ceramide moiety
Author(s) -
Nakakuma Hideki,
Arai Mitsuhiro,
Kawaguchi Tatsuya,
Horikawa Kentaro,
Hidaka Michihiro,
Sakamoto Kiyoshi,
Iwamori Masao,
Nagai Yoshitaka,
Takatsuki Kiyoshi
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81660-6
Subject(s) - glycosphingolipid , ceramide , moiety , epitope , glycolipid , monoclonal antibody , sphingosine , chemistry , biochemistry , galactose , fatty acid , conformational epitope , antibody , sphingolipid , stereochemistry , biology , receptor , immunology , apoptosis
A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin‐layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the 3 GalCers, the mAb reacted with two GalCers which contained α‐hydroxy fatty acids, but not with GalCer composed of nonhydroxy fatty acids. These findings suggest not only that the mAb discriminated the fatty acid composition in the ceramide moiety of GalCer, but also that the ceramide structure defines the immunological epitope as it is known to do for the carbohydrate moiety of glycosphingolipid.