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A special repressor/activator system controls distribution of mRNA between translationally active and inactive mRNPs in rabbit reticulocytes
Author(s) -
Minich Waldemar B.,
Korneyeva Nadezhda L.,
Berezin Yuri V.,
Ovchinnikov Lev P.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81659-x
Subject(s) - reticulocyte , polysome , ribosome , messenger rna , translation (biology) , protein biosynthesis , activator (genetics) , biology , microbiology and biotechnology , chemistry , biochemistry , gene , rna
Translation of free mRNPs and polyribosomal mRNPs from rabbit reticulocytes was studied in a rabbit reticulocyte and wheat germ cell‐free systems. It has been shown that the translation efficiency of polyribosomal mRNPs and the mRNA isolated from the particles is nearly the same in both systems. At the same time, free mRNPs' translatability, which is high in the homologous cell‐free system, is very low in the system from wheat germs. Translation efficiency of free mRNPs in the wheat germ system can be restored by addition of 0.5 M KCl‐wash of rabbit reticulocyte ribosomes. These results testify to the existence of some special repressor/activator system which controls the distribution of mRNA between free mRNPs and polyribosomes in rabbit reticulocytes.