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Stimulation of F A and phosphatase‐1 activities by insulin in 3T3‐L1 cells
Author(s) -
Villa-Moruzzi Emma
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81654-0
Subject(s) - phosphatase , insulin , activator (genetics) , stimulation , medicine , endocrinology , trypsin , alkaline phosphatase , chemistry , cytosol , insulin receptor , enzyme activator , biology , enzyme , biochemistry , insulin resistance , receptor
The phosphatase‐1 activator F A and phosphatase‐1 were assayed in 3T3‐L1 cells exposed to insulin. The cytosolic F A activity was transiently stimulated (7‐8‐fold) 1 and 2 min after exposure to 10 −8 M insulin and returned to control values within 5–10 min. Cytosolic phosphatase‐1 (assayed after trypsin treatment) was activated (120–140% of controls) between 2 and 5 min and returned to control values within 10 min. Insulin effects were dose‐dependent, with maximum stimulation of both activities at 10 −8 M insulin. The possibility that F A and other kinases mediate phosphatase activation by insulin is discussed.