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Guanine nucleotides modify calcium entry induced by insulin‐like growth factor‐I
Author(s) -
Kojima Itaru,
Kitaoka Masafumi,
Ogata Etsuro
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81637-0
Subject(s) - calcium , gtp' , chemistry , nucleotide , microbiology and biotechnology , biophysics , guanine , biochemistry , endocrinology , biology , enzyme , organic chemistry , gene
When calcium influx rate was measured in the cell suspension system instead of confluent monolayer culture, IGF‐I (1 nM) augmented calcium entry independent of the cell cycle. In a cell suspension system, NaF increased calcium entry in a dose‐dependent manner, which was sensitive to tetramethrin. When GTP‐γS was introduced into the cells by employing ATP 4− , calcium influx rate was elevated whereas ATP‐γS was without effect. In cells loaded with GDP‐βS, IGF‐I did not stimulate calcium entry. These results suggest that a G‐protein is involved in the signal transduction of IGF‐I action.