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Sequence and expression of a novel GABA A receptor α subunit
Author(s) -
Ymer Sanie,
Draguhn Andreas,
Köhler Martin,
Schoneid Peter R.,
Seeburg Peter H.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81630-8
Subject(s) - complementary dna , protein subunit , gabaa receptor , xenopus , long term potentiation , cerebellum , microbiology and biotechnology , messenger rna , hippocampus , biology , hippocampal formation , receptor , peptide sequence , amino acid , biochemistry , chemistry , endocrinology , gene
Cloned cDNA encoding the bovine α 4 subunit of the GABA a receptor has been isolated. The predicted 521 amino acid long mature protein contains an exceptionally long intracellular domain and shares 53–56% sequence similarity to the previously characterized α 1 , α 2 and α 3 , subunits. Co‐expression of α 4 and β 1 in Xenopus oocytes resulted in the formation of GABA‐gated chloride channels with expected pharmacology, although no benzodiazepine potentiation was observed. Northern analysis indicates that a 4 kb α 4 mRNA is expressed in the calf cerebellum, cortex and hippocampus but is barely detectable in the rat brain.