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Papaya proteinase IV amino acid sequence
Author(s) -
Ritonja Anka,
Buttle David J.,
Rawlings Neil D.,
Turk Vito,
Barrett Alan J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81627-8
Subject(s) - papain , carica , chymopapain , peptide sequence , biochemistry , cysteine , amino acid residue , enzyme , sequence (biology) , superfamily , cysteine proteinase inhibitors , amino acid , biology , sequence alignment , chemistry , gene , botany , apoptosis , programmed cell death , caspase
The amino acid sequence of papaya proteinase IV (PPIV), a major proteinase from the latex of Carica papaya [(1989) Biochem. J. 261, 469‐476] is described. The enzyme has a high degree of sequence identity with papaya proteinase III, chymopapain and papain (81, 70 and 67%, respectively), and is clearly a member of the papain superfamily of cysteine proteinases. Nevertheless, the sequence shows substitution of certain residues conserved in all other known members of the superfamily. It is suggested that some of these substitutions may account for the unusual specificity of PPIV.