Premium
Regulation of glutathione S‐transferase subunits 3 and 4 in cultured rat hepatocytes
Author(s) -
Morel Fabrice,
Vandenberghe Yves,
Pemble Sally,
Taylor John B.,
Ratanasavanh Damrong,
Rogiers Vera,
Ketterer Brian,
Guillouzo André
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81624-2
Subject(s) - glutathione s transferase , glutathione , messenger rna , microbiology and biotechnology , protein subunit , cell culture , nicotinamide , hepatocyte , biology , complementary dna , phenobarbital , chemistry , biochemistry , enzyme , in vitro , endocrinology , gene , genetics
mRNA levels of glutathione S‐transferase (GST) subunits 3 and 4 were measured with a specific cDNA probe in adult rat hepatocytes maintained either in conventional culture or in coculture with rat liver epithelial cells. Four media conditions were used, i.e. with or without fetal calf serum (FCS) and with nicotinamide or dimethylsulfoxide (DMSO). When FCS was present in the culture medium, GST subunit 3 and 4 mRNAs were expressed at a level close to that found in freshly isolated hepatocytes during the whole culture period both in conventional culture and in coculture. All other culture conditions resulted in an increase of GST 3 and 4 mRNA levels. After exposure to phenobarbital an increase in GST 3 and 4 mRNA levels was demonstrated in both culture systems. Comparison with previous findings on the expression of GST subunits 1, 2 and 7 in the same culture conditions indicates that the different classes of GST are regulated independently.