Premium
Molecular cloning and characterization of the complementary DNA coding for the B‐chain of murine Clq
Author(s) -
Petry Franz,
Reid Kenneth B.M.,
Loos Michael
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81622-9
Subject(s) - microbiology and biotechnology , complementary dna , northern blot , rna , biology , coding region , spleen , cdna library , dna , messenger rna , southern blot , molecular cloning , gene , genetics , immunology
cDNA clones coding for the B‐chain of murine Clq were isolated from a mouse macrophage library. The characterized clones include the total coding region plus a leader sequence. High homology was found with human Clq B‐chain in the coding region (81%). Northern blot analysis of total RNA from different tissues of Balb/c mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of thioglycolate‐activated peritoneal macrophages. The probe also hybridized with mRNA from spleen, thymus and heart. Extremely weak signals were found in liver, kidney, lung and intestine tissues.