Premium
RNA editing at a splicing site of NADH dehydrogenase subunit IV gene transcript in wheat mitochondria
Author(s) -
Lamattina Lorenzo,
Weil Jacques-Henry,
Grienenberger Jean-Michel
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81620-5
Subject(s) - nadh dehydrogenase , gene , biology , genetics , rna splicing , open reading frame , protein subunit , complementary dna , stop codon , mitochondrial dna , microbiology and biotechnology , peptide sequence , rna , biochemistry
Comparison between the sequence of the gene coding for the wheat mitochondrial NADH dehydrogenase subunit IV (nad4) and the cDNA sequence obtained by reverse transcription, using total wheat mtRNA as template, has shown the presence of a uridine residue, not encoded by the genomic sequence, at the exon2‐exon3 junction of the spliced transcript. This U creates a non‐encoded CUG leucine codon which is essential for maintaining the reading frame, as shown by the conservation of the amino acid sequence of the NAD4 protein in various species. The addition of a U or the specific post‐transcriptional conversion of a C to a U could explain this phenomenon.