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Structure of spheroidene in the photosynthetic reaction center from Y Rhodobacter sphaeroides
Author(s) -
Arnoux B.,
Ducruix A.,
Reiss-Husson F.,
Lutz M.,
Norris J.,
Schiffer M.,
Chang C.-H.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81612-6
Subject(s) - rhodobacter sphaeroides , photosynthetic reaction centre , photosynthesis , chemistry , bacteriochlorophyll , rhodospirillaceae , rhodospirillales , photochemistry , biophysics , biochemistry , biology
The structure of the reaction center of Y Rhodobacter sphaeroides has been solved at 3 Å resolution, using the atomic coordinates of the reaction center from the carotenoidless mutant R26 Rhodobacter sphaeroides . The structure has been refined by a simulated annealing with the computer program X‐PLOR, leading to a crystallographic R factor of 0.22 using reflections between 8 and 3 Å. The spheroidene molecule which is bound to the Y reaction center has been fitted in the electron density map as a 15‐ cis isomer with a highly asymmetric structure. The cis ‐bond is located at proximity from ring I of the accessory bacteriochlorophyll on the inactive M side. The nature of the cis ‐bond was confirmed by resonance Raman spectra obtained from Y reaction center crystals. The structure of spheroidene in Y reaction center is compared to that proposed for 1,2‐dihydroneurosporene in Rhodopseudomonas viridis reaction center crystals.