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Retinoic acid controls expression of epidermal transglutaminase at the pre‐translational level
Author(s) -
Michel Serge,
Reichert Uwe,
Isnard Jean Luc,
Shroot Braham,
Schmidt Rainer
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81609-6
Subject(s) - retinoic acid , tissue transglutaminase , immunoprecipitation , chemistry , monoclonal antibody , in vitro , microbiology and biotechnology , immunofluorescence , tretinoin , biochemistry , enzyme , biology , antibody , immunology , gene
Human epidermal keratinocytes were cultured until sub‐confluence in low Ca 2+ (0.15 mM) serum‐free synthetic MCDB 153 medium. Raising the Ca 2+ concentration to 1.15 mM caused an increase in envelope competence as well as plasma membrane associated transglutaminase (TGm) activity. This increase was not observed when the high Ca 2+ medium contained retinoic acid. Immunofluorescence studies as well as immunoblotting with the TGm‐specific monoclonal antibody B.C1 revealed that retinoic acid inhibits expression of TGm. Isolation and in vitro translation of mRNA with subsequent immunoprecipitation showed that retinoic acid inhibits TGm expression at the pretranslational level.