Premium
Protective effect of lipoproteins containing apoprotein A‐I on Cu 2+ ‐ catalyzed oxidation of human low density lipoprotein
Author(s) -
Ohta Takao,
Takata Kyoko,
Horiuchi Seikoh,
Morino Yoshimasa,
Matsuda Ichiro
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81590-x
Subject(s) - chemistry , lipoprotein , thiobarbituric acid , low density lipoprotein , in vitro , catalysis , fragmentation (computing) , biochemistry , apolipoprotein b , high density lipoprotein , cholesterol , antioxidant , lipid peroxidation , biology , ecology
Two apoprotein A‐I (apoA‐I)‐containing lipoproteins, one containing apoA‐I and apoA‐II (LpA‐I/A‐II) and the other containing only apoA‐I (LpA‐I), were examined for their effect on Cu 2+ ‐mediated oxidation of low density lipoprotein (LDL). The presence of LpA‐I or LpA‐I/A‐II prevented LDL oxidation when assessed by the electrophoretic mobility, apoprotein B fragmentation and amounts of thiobarbituric acid‐reactive substances. The protection of LDL oxidation by these lipoproteins was effective for up to 6 h, with LpA‐I being more active than LpA‐I/A‐II. Results from these in vitro model experiments raise a possibility that LpA‐I mayplay a role in protecting LDL from Cu 2+ ‐mediated oxidation.