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P‐glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated
Author(s) -
Greenberger Lee M.,
Croop James M.,
Horwitz Susan Band,
Arceci Robert J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81586-8
Subject(s) - glycoprotein , p glycoprotein , multiple drug resistance , glycosylation , gene , biology , endometrium , cell culture , gene product , microbiology and biotechnology , uterus , cancer research , gene expression , genetics , endocrinology , drug resistance
There are 3 members of the multidrug‐resitance gene family expressed in mouse. Only one of these, mdr lb , and its gene product P‐glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P‐glycoprotein in the gravid uterus is significantly larger ( M r 155000) compared to P‐glycoprotein encoded by mdr lb in a murine multidrug‐resistant cell line ( M r 140000). However, both species co‐migrate after enzymatic removal of N ‐linked sugars ( M r 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P‐glycoprotein from normal and multidrug‐resistant cells.