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Insulin stimulates the translocation of protein kinase C in rat adipocytes
Author(s) -
Ishizuka Tatsuo,
Cooper Denise R.,
Farese Robert V.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81565-0
Subject(s) - cytosol , protein kinase a , insulin , chromosomal translocation , mitogen activated protein kinase kinase , protein kinase c , chemistry , kinase , biochemistry , medicine , biology , microbiology and biotechnology , endocrinology , enzyme , gene
Insulin‐induced changes in protein kinase C were examined in cytosol and membrane fractions of rat adipocytes enzymatically after Mono Q column chromatography and by immunoblotting. During a 5–20 min period of insulin treatment, cytosolic protein kinase C decreased by approximately 50%, whereas membrane protein kinase C increased nearly 2‐fold. These findings suggest that insulin stimulates the translocation of protein kinase C in rat adipocytes.