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An antiserum to the extracellular domain of the Alzheimer amyloid precursor recognizes 70 and 88 kDa brain proteins
Author(s) -
Simpson James,
Bladon Christine M.,
Yates Celia M.,
Harmar Anthony J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81542-x
Subject(s) - antiserum , extracellular , blot , amyloid precursor protein , amyloid (mycology) , biochemistry , gel electrophoresis , chemistry , human brain , microbiology and biotechnology , biology , antibody , alzheimer's disease , pathology , medicine , immunology , neuroscience , gene , inorganic chemistry , disease
An antiserum raised to the extracellular domain (residues 556–566) of the Alzheimer amyloid precursor protein recognized 70 and 88 kDa proteins in Western blots of rat, Alzheimer, Down's syndrome and control human brain separated by SDS‐PAGE. The 70 kDa protein band was resolved into 5 spots by two‐dimensional electrophoresis. The findings provide further evidence that a 70 kDa protein is a metabolite of the amyloid precursor protein and reveal an 88 kDa protein which was reduced in 3 out of 6 brains with Alzheimer pathology.