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A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II
Author(s) -
Noda Masaharu,
Suzuki Harukazu,
Numa Shosaku,
Stühmer Walter
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81531-5
Subject(s) - tetrodotoxin , sodium channel , xenopus , point mutation , saxitoxin , chemistry , mutation , biophysics , complementary dna , microbiology and biotechnology , patch clamp , sodium , biology , biochemistry , gene , toxin , organic chemistry , receptor
A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage‐clamp in Xenopus oocytes injected with the corresponding cDNA‐derived mRNA.