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S‐phase inhibitors induce vimentin expression in human promonocytic U‐937 cells
Author(s) -
Mata F.,
Rius C.,
Cabañas C.,
Bernabeu C.,
Aller P.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81521-2
Subject(s) - vimentin , microbiology and biotechnology , biology , northern blot , immunofluorescence , gene expression , messenger rna , cellular differentiation , gene , antibody , immunohistochemistry , biochemistry , immunology
The administration of hydroxyurea (3 × 10 −4 M) and cytosine arabinoside (10 −7 M) greatly induces the expression of the vimentin gene in human promonocytic leukemia U‐937 cells. The induction takes place at both the mRNA and protein levels, as demonstrated by Northern blot, immunoblot and immunofluorescence assays. On the contrary, the drugs inhibit the expression of c‐ myc and omithine decarboxylase, and do not modify significantly the expression of β‐actin. Since hydroxyurea and cytosine arabinoside trigger the phenotypic differentiation of U‐937 cells, as demonstrated by the induction of the differentiation‐specific CDllb and CDllc antigens, it is concluded that vimentin expression might be implicated in the maturation of these cells.