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Lipoxins are major lipoxygenase products of rainbow trout macrophages
Author(s) -
Pettitt Trevor R.,
Rowley Andrew F.,
Secombes Chris J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81520-0
Subject(s) - lipoxygenase , chemistry , rainbow trout , ionophore , biochemistry , lipoxin , chromatography , enzyme , fish <actinopterygii> , biology , membrane , receptor , fishery
Rainbow trout macrophages synthesize lipoxins as major lipoxygenase products entirely from endogenous fatty acids. High‐performance liquid Chromatographic analysis of the supernatants from macrophages challenged with calcium ionophore A23187 revealed a range of lipoxygenase products including mono‐hydroxy fatty acids, leukotrienes B 4 and B 5 and four major peaks with retention times and UV spectra characteristic of lipoxins (λ max 302 nm). Cochromatography with authentic standards, UV spectroscopy and radiolabelling with ( 14 C]arachidonate and eicosapentaenoate allowed tentative identification of the two largest peaks as lipoxin A 4 and A 5 .