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Yeast vacuolar aminopeptidase yscI
Author(s) -
Cueva Rosario,
García-Alvarez Nieves,
Suárez-Rendueles Paz
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81510-8
Subject(s) - aminopeptidase , open reading frame , yeast , saccharomyces cerevisiae , gene , locus (genetics) , biology , nucleic acid sequence , biochemistry , microbiology and biotechnology , amino acid , peptide sequence , leucine
The structural gene, APE1, (LAP4), for the vacuolar aminopeptidase I of Saccharomyces cerevisiae was cloned with the aid of a staining technique which permitted monitoring of aminopeptidase activity in yeast colonies. Genetic linkage data demonstrate that integrated copies of the cloned gene map to the APEI locus. The nucleotide sequence of the cloned gene was determined. The open reading frame of APEI consists of 514 codons and, therefore, encodes a larger protein (MW 57 003) than the reported mature aminopeptidase yscI (MW 44 800), suggesting that proteolytic processing must occur. A 1.75‐kb mRNA, which is made in substantial amounts only when yeast cells have exhausted the glucose supply, was identified.