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Evidence for leukotriene C 4 transport mediated by an ATP‐dependent glutathione S‐conjugate carrier in rat heart and liver plasma membranes
Author(s) -
Ishikawa Toshihisa,
Kobayashi Kazuo,
Sogame Yoshihisa,
Hayashi Koichiro
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81503-0
Subject(s) - conjugate , glutathione , chemistry , membrane , plasma , plasma chemistry , leukotriene c4 , biochemistry , biophysics , leukotriene , medicine , biology , enzyme , physics , mathematical analysis , mathematics , asthma , quantum mechanics
Using rat heart sarcolemma and liver plasma membrane vesicles, it has been verified that the transport of leukotriene C 4 (LTC 4 ) across membranes is an ATP‐dependent process; the apparent K m for LTC 4 was 150 nM (heart sarcolemma) or 250 nM (liver plasma membrane). S‐(2,4‐dinitrophenyl)‐glutathione (DNP‐SG) inhibited LTC 4 uptake into the vesicles dose‐dependently ( I 50 = 25 (μM for both heart sarcolemma and liver plasma membrane vesicles). Mutual inhibition between LTC 4 and DNP‐SG in uptake into the vesicles demonstrates that transport of LTC 4 is mediated by an ATP‐dependent glutathione S‐conjugate carrier.