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Histamine and bradykinin stimulate the phosphoinositide turnover in human umbilical vein endothelial cells via different G‐proteins
Author(s) -
Voyno-Yasenetskaya T.A.,
Panchenko M.P.,
Nupenko E.V.,
Rybin V.O.,
Tkachuk V.A.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81496-6
Subject(s) - bradykinin , umbilical vein , histamine , chemistry , microbiology and biotechnology , medicine , biochemistry , endocrinology , biology , receptor , in vitro
The G‐proteins which regulate hormonal turnover of phosphoinositide (PI) in human umbilical vein endothelial cells have been investigated. A 40–41 kDa doublet present in the membranes of these cells was selectively ADP ribosylated by pertussis toxin (PTx), and this doublet was G α 2 and G α 3 according to immunoblotting with specific antisera. By contrast, a doublet of 24–26 kDa proteins in the same membrane preparations was ADP ribosylated by the C 3 component of botulinum toxin (BoTx). PTx‐dependent ADP ribosylation blocked stimulation of PI turnover by histamine, but did not affect stimulation by bradykinin, whereas BoTx (C 2 + C 3 components) had the opposite effect. Thus two different groups of G‐proteins may be involved in hormone‐dependent stimulation of PI turnover in human umbilical vein endothelial cells.