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The in vivo phosphorylation sites of bovine αB‐crystallin
Author(s) -
Voorter Christina E.M.,
de Haard-Hoekman Willeke A.,
Roersma Eric S.,
Meyer Helmut E.,
Bloemendal Hans,
de Jong Wilfried W.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81491-7
Subject(s) - phosphorylation , in vivo , chemistry , microbiology and biotechnology , biochemistry , biology , genetics
Phosphate content determinations established that in αB‐crystallin two phosphate groups can be present in vivo in bovine lenses. Comparison of tryptic digests of phosphorylated and unphosphorylated αB chains, revealed the location of the two phosphorylation sites in tryptic peptides T2 and T3. Thermolytic digestion and gas‐phase sequencing demonstrated that Ser‐19 and Ser‐45 are the in vivo phosphorylation sites of bovine αB‐crystallin. This pattern of phosphorylation differs from the previously reported in vitro obtained results.