Premium
The hexose carrier from Chlorella
Author(s) -
Sauer Norbert,
Tanner Widmar
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81489-9
Subject(s) - cotransporter , hexose , biochemistry , open reading frame , complementary dna , pentose , glut1 , symporter , chlorella , chemistry , glucose transporter , biology , transporter , microbiology and biotechnology , gene , peptide sequence , enzyme , algae , ecology , organic chemistry , endocrinology , fermentation , insulin , sodium
The cDNA coding for the inducible H + /hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose‐induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to induction; it is not expressed in a hexose transport mutant. An open reading frame allows for a membrane protein of 533 amino acids with a relative molecular mass of 57 kDa. This protein is highly homologous to the human and rat glucose transporters catalyzing facilitated diffusion and to the bacterial H + /pentose cotransporters. It is not related to the H + /lactose cotransporter of E.colli and to the mammalian Na + /glucose cotransporter.