Premium
A patch‐clamp study of ion channels of inner and outer membranes and of contact zones of E. coli , fused into giant liposomes
Author(s) -
Berrier Catherine,
Coulombe Alain,
Houssin Christine,
Ghazi Alexandre
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81486-3
Subject(s) - membrane , bacterial outer membrane , biophysics , ion channel , patch clamp , chemistry , liposome , clamp , barrel (horology) , inner membrane , escherichia coli , materials science , biology , biochemistry , composite material , mechanical engineering , clamping , receptor , engineering , gene
Inner and outer membranes of Escherichia coli and contact zones were isolated and fused separately with giant liposomes amenable to patch‐clamp recording. Different types of large pressure‐activated channels were localized in the inner membrane fraction which also contained smaller, pressure‐insensitive channels. The outer membrane contained pressure‐insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones.